CYP26B1 Knockout cell line(KYSE-30)

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location： Home > Products > KO Cell Lines > CYP26B1 Knockout cell line(KYSE-30)

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CYP26B1 Knockout cell line(KYSE-30) Order

CYP26B1 Knockout cell line(KYSE-30)
Catalog#
YKO-HJ033
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CYP26B1 Knockout cell line(KYSE-30)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CYP26B1 Knockout cell line (KYSE-30)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
CYP26B1
Gene ID
56603
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 3 (based on transcript CYP26B1-201)
Validation: PCR+Sanger Sequencing
CYP26B1 Knockout cell line(KYSE-30) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:KYSE-30		 STR Info (Cell bank)
Cell Line:KYSE-30
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 9999
D7S820 11121112
D16S539 10121012
VWA 1619161819.0
TH01 9999
AMEL XXXX
TPOX 9999
CSF1PO 10101010
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CYP26B1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the KYSE-30 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CYP26B1 gene knockout CYP26B1 Knockout cell line (KYSE-30) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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